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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as items (Fig. 9) - spectrophotometers. As drawn in Fig - https://www.quora.com/profile/Julie-Ann-DeSa-Lorenz. 9, the reaction might also involve electron transfer from H4B to the Fe, IIO2 intermediate


l9 As displayed in Fig. 9, this might be due to the fact that the H4B extreme acts as an electron sink in a later point in the reaction, so as to enable NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.